1、个人简介

黄丽，博士，研究员，博士生导师，国家自然科学基金优秀青年基金获得者。任农业部经济动物疫病重点实验室学术委员、中国生物化学与分子生物学会农业生物化学与分子生物学分会副秘书长、畜牧兽医学会兽医传染病学分会青年委员、黑龙江省蛋白质组学学会理事、黑龙江省细胞生物学会青年委员、黑龙江省青年科技工作者协会会员、黑龙江省兽医免疫学重点实验室学术带头人、Frontiers in Immunology、Microorganisms、Animals等期刊客座主编、中国农业科学杂志青年编委、Animal Diseases杂志科学编辑；获得“哈尔滨市三八红旗手”荣誉称号。主要从事非洲猪瘟病毒、猪伪狂犬病病毒等重要动物病毒逃逸宿主天然免疫机制研究。主持国家自然科学基金优秀青年基金项目、国家自然科学基金面上项目、国家重点研发计划子课题、黑龙江省优秀青年基金等项目。在Cell Reports（3篇）、Journal of Virology（5篇）、Journal of Immunology（2篇）、Journal of Biological Chemistry（1篇）等杂志发表第一作者或者通讯作者（含共同）科技论文20余篇；研发非洲猪瘟病毒ELISA抗体检测试剂盒2个，获得国家发明专利1项、中华农业科技奖1项。

2、联系方式

电话：0451-51051759

邮箱：highlight0315@163.com

通讯地址：黑龙江省哈尔滨市香坊区哈平路678号

3、教育背景

2007.09-2012.06，南京农业大学动物医学院 预防兽医学 博士

2003.09-2007.09，山东农业大学动物医学院 预防兽医学 本科

4、工作经历

2012.07-2016.12，中国农业科学院哈尔滨兽医研究所 助理研究员

2017.01-2021.12，中国农业科学院哈尔滨兽医研究所 基础免疫创新团队 副研究员

2022.01至今，中国农业科学院哈尔滨兽医研究所 基础免疫创新团队 研究员

5、承担主要科研项目

1.国家自然科学基金优秀青年基金项目：兽医免疫学（32322081，2024.01-2026.12） 200万元，主持；

2.国家自然科学基金面上项目：波形蛋白识别非洲猪瘟病毒DNA激活NLRP3炎症小体的分子机制（32270156，2023.01-2026.12）54万元，主持；

3.国家十四五重点研发计划“非洲猪瘟亚单位疫苗创制”重点专项子课题：ASF亚单位疫苗的TCR和BCR免疫组库模型建立与验证（2021YFD1801300，2021.12-2024.11）100万元，主持；

4.黑龙江省自然科学基金优秀青年项目：非洲猪瘟病毒pI215L蛋白抑制I型干扰素产生的分子机制（YQ2020C022，2020.07-2023.07）10万元，主持；

5.国家十三五重点研发计划子课题：生物安全关键技术研发重点专项2018年度项目（2018YFC1200902, 2018.06-2021.06）50万元，主持；

6.国家自然科学基金专项项目：非洲猪瘟病毒感染与致病机制（31941002，2019.07-2022.06）300万元，参与；

7.国家自然科学基金青年基金：20S蛋白酶体亚基在脑心肌炎病毒复制与组装中的作用（31300139，2014.01-2016.12）23万元，主持。

6、代表性文章

1. Guangqiang Ye, Zhaoxia Zhang, Xiaohong Liu, Hongyang Liu, Weiye Chen, Chunying Feng, Jiangnan Li, Qiongqiong Zhou, Dongming Zhao, Shuai Zhang, Hefeng Chen, Zhigao Bu, Li Huang*, Changjiang Weng*, African swine fever virus pH240R enhances viral replication via inhibition of the type I IFN signaling pathway. Journal of Virology. 2024 Feb 14:e0183423.

2. Jiangnan Li , Jie Song, Shijun Zhou, Shuai Li, Jia Liu , Tingting Li, Zhaoxia Zhang, Xianfeng Zhang, Xijun He, Weiye Chen, Jun Zheng, Dongming Zhao, Zhigao Bu, Li Huang*, Changjiang Weng*,  Development of a new effective African swine fever virus vaccine candidate by deletion of the H240R and MGF505-7R genes results in protective immunity against the Eurasia strain. Journal of Virology. 2023 Sep 28:e0070423.  

3. Guangqiang Ye, Hongyang Liu, Xiaohong Liu, Weiye Chen, Jiangnan Li, Dongming Zhao, Gang Wang, Chunying Feng, Zhaoxia Zhang, Qiongqiong Zhou, Jun Zheng, Zhigao Bu, Changjiang Weng, Li Huang, African Swine Fever Virus H240R Protein Inhibits the Production of Type I Interferon through Disrupting the Oligomerization of STING. Journal of Virology. 2023; 97(9): e0057723.

4. Li Huang, Weiye Chena, Hongyang Liua, Mengdi Xuea, Siqi Dong, Xiaohong Liu, Chunying Feng, Shinuo Cao, Guangqiang Ye, Qiongqiong Zhou, Zhaoxia Zhang, Jun Zheng, Jiangnan Li, Dongming Zhao, Zilong Wang, Encheng Sun, Hefeng Chen, Shuai Zhang, Xue Wang, Xianfeng Zhang, Xijun H, Yuntao Guan, Zhigao Bu*, Changjiang Weng*, African Swine Fever Virus HLJ/18 CD2v Suppresses Type I IFN Production and IFN-Stimulated Genes Expression through Negatively Regulating cGMP-AMP Synthase STING and IFN Signaling Pathways. Journal of Immunology. 2023, 210 (9): 1338-1350.

5. Qiongqiong Zhoua, Longfeng Zhanga, Qihong Lin, Hongyang Liu, Guangqiang Ye, Xiaohong Liu, Shuang Jiao, Jiangnan Li, Yandong Tang, Deshi Shi, Li Huang*, Changjiang Weng*, Mechanism of PRV infection activating TLRs-NF-kB axis and AIM2 inflammasome to enhance inflammatory response. Journal of Virology. 2023, 97 (3).

6. Li Huang, Hongyang Liu, Guangqiang Ye, Xiaohong Liu, Weiye Chen, Zilong Wang, Shijun Zhou, Dongming Zhao, Zhaoxia Zhang, Chunying Feng, Xianfeng Zhang, Xijun He, Jun Zheng, Zhigao Bu*, Jiangnan Li*, Changjiang Weng*, ASFV pH240R affects the virulence of African swine fever virus by inhibiting type I interferon signaling and NLRP3-mediated inflammatory responses. Journal of Virology. 2023, 97 (2).

7. Hongyang Liu1, Guangqiang Ye1, Xiaohong Liu, Mengdi Xue, Qiongqiong Zhou, Longfeng Zhang, Kunli Zhang, Li Huang*, Changjiang Weng*. Vimentin inhibits type I interferon production by disrupting the TBK1-IKKε-IRF3 axis. Cell Reports. 2022, 41, 111469.

8. Xiaohong Liu, Hongyang Liu, Guangqiang Ye, Mengdi Xue, Huibin Yu, Chunying Feng, Qiongqiong Zhou, Xuemin Liu, Longfeng Zhang, Shuang Jiao, Changjiang Weng*, Li Huang*. African swine fever virus pE301R negatively regulates cGAS-STING signaling pathway by inhibiting the nuclear translocation of IRF3. Veterinary Microbiology. 274 (2022) 109556.

9. Qiongqiong Zhou, Longfeng Zhang, Hongyang Liu, Guangqian Ye, Li Huang*, Changjiang Weng*. Isolation and Characterization of Two Pseudorabies Virus and Evaluation of Their Effects on Host Natural Immune Responses and Pathogenicity. Viruses, 2022 14 (4): 712.

10. Li Huang, Jiangnan Li, Jun Zheng, Dan Li, Changjiang Weng, Multifunctional pMGF505-7R is a key virulence-related factor of African Swine Fever Virus. Frontiers in Microbiology, 2022. 852431.

11. Li Huang¹, Wenjie Xu¹, Hongyang Liu, Mengdi Xue, Xiaohong Liu, Kunli Zhang, Liang Hu, Jiangnan Li, Xuemin Liu, Zhida Xiang, Jun Zheng, Changyao Li, Weiye Chen, Zhigao Bu, Tao Xiong*, and Changjiang Weng*, African Swine Fever Virus pI215L Negatively Regulates cGAS-STING Signaling Pathway through Recruiting RNF138 toInhibit K63-Linked Ubiquitination of TBK1. Journal of Immunology, 2021 207(11): 2754-2769.

12. Jiangnan Li1, Jie Song1, Li Kang, Li Huang, Shijun Zhou, Liang Hu, Jun Zheng, Changyao Li, Xianfeng Zhang, Xijun He, Dongming Zhao, Zhigao Bu, Changjiang Weng, pMGF505-7R determines pathogenicity of African swine fever virus infection by inhibiting IL-1β and type I IFN production. PLoS Pathogens. 2021 Jul 26; 17(7):e1009733.

13. Li Huang#, Hongyang Liu#, Kunli Zhang, Qingwen Meng, Liang Hu, Yuanfeng Zhang, Zhida Xiang, Jiangnan Li, Yuying Yang, Yali Chen, Shangjin Cui, Hong Tang, Huadong Pei, Zhigao Bu, and Changjiang Weng, Ubiquitin-Conjugating Enzyme 2S Enhances Viral Replication by Inhibiting Type I IFN Production through Recruiting USP15 to Deubiquitinate TBK1. Cell Reports. 2020, 32, 108044.

14. Kunli Zhang, Yuanfeng Zhang, Juan Xue, Qingwen Meng, Hongyang Liu, Caihong Bi, Changyao Li, Liang Hu, Huibin Yu, Tao Xiong, Yuying Yang, Shangjin Cui, Zhigao Bu, Xijun He, Jiangnan Li, Li Huang*, Changjiang Weng*. DDX19 inhibits type I interferon production by disrupting TBK1-IKKε-IRF3 interactions and promoting TBK1 and IKKε degradation. Cell Reports. 2019 26: 1258-1272.

15. Li Huang, Tao Xiong, Huibin Yu, Quan Zhang, Kunli Zhang, Changyao Li, Liang Hu, Yuanfeng Zhang, Lijie Zhang, Qinfang Liu, Shengnan Wang, Xijun He, Zhigao Bu, Xuehui Cai, Shangjin Cui, Jiangnan Li and Changjiang Weng*. Encephalomyocarditis virus 3C protease attenuates type I interferon production through disrupting the TANK-TBK1-IKKε-IRF3 complex. Biochemical Journal. 2017 474: 2051-2065.

16. Jiangnan Li, Shengnan Wang, Li Huang*, Changjiang Weng*. Secondary Haemophilus parasuis infection enhances highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) infection-mediated inflammatory responses. Veterinary Microbiology. 2017, 204: 35-42.

17. Huibin Yu¹, Li Huang¹, Yuanfeng Zhang, Liang Hu, Shengnan Wang, Jiangnan Li, Xuehui Cai, Shangjin Cui, Changjiang Weng. An attenuated EMCV-HB10 strain acts as a live viral vector delivering a foreign gene. Journal of General Virology. 2016 Sep; 97(9):2280-90.

18. Li Huang, Qinfang Liu, Lijie Zhang, Quan Zhang, Liang Hu, Changyao Li, Shengnan Wang, Jiangnan Li, Yuanfeng Zhang, Huibin Yu, Yan Wang, Zhaohua Zhong, Tao Xiong, Xueshan Xia, Xiaojun Wang, Li Yu, Guohua Deng, Xuehui Cai, Shangjin Cui, Changjiang Weng*. Encephalomyocarditis Virus 3C Protease relieves TANK Inhibitory Effect on TRAF6-mediated NF-kappaB Signaling through Cleavage of TANK, Journal of Biological Chemistry, 2015, 290(46): 27618-32.

19. Jiangnan Li, Liang Hu, Yuanyuan Liu, Li Huang, Yang Mu, Xuehui Cai, Changjiang Weng*, DDX19A Senses Viral RNA and Mediates NLRP3-Dependent Inflammasome Activation, Journal of Immunology, 2015.12.15, 195(12): 5732-5749

20. Jiangnan Li, Dongwei, Guo, Li Huang, Manman Yin, Qingfang Liu, Yan Wang, Chunmei Yang, Yuanyuan Liu, Lijie Zhang, Zhijun Tian, Xuehui Cai, Liyun Yu, Changjiang Weng*, The interaction between host Annexin A2 and viral Nsp9 is beneficial for replication of porcine reproductive and respiratory syndrome virus, Virus Research, 2014, 30(189): 106-113.

21. Li Huang, Ruibing Cao, Ning Wang, Ke Liu, Jianchao Wei, Hassan Isahga, Lijie Song Weiyong Zuo, Bin Zhou, Weiwu Wang, Xiang Mao*, Puyan Chen*. The design and Recombinant Protein expression of a Consensus Porcine Interferon: CoPoIFN-α, Cytokine, 2012, 57(1): 37-45.

22. Li Huang, Xiang Mao*, Najmoutin G. Abdulaev, Tony Ngo, Wei Liu, and Kevin D. Ridge. One-step Purification of a Functional, Constitutively Activated Form of Visual Arrestin, Protein expression and purification, 2012, 82(1): 55-60.

23. Ming-xia Sun, Li Huang, Rui Wang, Ya-ling Yu, Chen Li, Peng-peng Li, Xiao-chun Hu, Hong-ping Hao, Hassan. A. shag, Xiang Mao. Porcine Reproductive and Respiratory syndrome Virus Induce Autophagy to Promote Virus Replication. Autophagy. 2012, 8:1434-1447.

24. Li Huang, Yuanpeng Zhang, Yaling Yu, Mingxia Sun, Chen Li, Bo Ni, Xiuhua Wang, Puyan Chen, Xiang Mao*. Role of cholesterol in porcine reproductive and respiratory syndrome virus (PRRSV) infection on MARC-145 cells. Biochemical and Biophysical Research Communications. 2011, 414(3): 545-50.

7、专利  

黄丽，陈溥言，侯凤香，刘珂，曹瑞兵。一种复合猪α干扰素基因及其重组载体，ZL201010125057.6，发明专利，中国。