中国农业科学院哈尔滨兽医研究所

1、个人简历

翁长江，博士，研究员，博士生导师，中国农业科学院哈尔滨兽医研究所基础免疫创新团队首席科学家，中国农业科学院领军人才，黑龙江省兽医免疫学重点实验室主任。主要从事非洲猪瘟病毒(ASFV)、猪繁殖与呼吸综合征病毒(PRRSV)等感染、免疫逃逸和致病机制的研究。近年来，主持国家自然科学基金委非洲猪瘟重点专项、地区联合创新基金、面上项目、中国农业科学院知识创新工程和科技部“十四五”重点研发计划项目。近五年在Cell Rep、PLoS Pathog、J Biol Chem、J Immunol等杂志发表科技论文50余篇，被国内外同行引用500余次。中国细胞生物学学会免疫细胞学分会理事、中国免疫学会兽医免疫学分会理事和全国农业生物化学与分子生物学学会理事。先后担任昆明理工大学、长江大学、浙江农林科技大学、沈阳农业大学和吉林农业大学客座教授。获得中华农业科技奖1项。

2、联系方式

电话：0451-51051756

邮件：wengchangjiang@caas.cn; wengchji@163.com

通讯地址：黑龙江省哈尔滨市香坊区哈平路678号 150069

3、教育背景

2000.09-2005.10 中国科学院/中国科学院研究生院微生物研究所博士

1996.09-1999.07 华中农业大学动物医学院硕士

1991.09-1994.07 湖北农学院畜牧兽医专业（长江大学动物医学系）

4、工作经历

2016.07-今哈尔滨兽医研究所基础免疫团队首席科学家

2010.10-2016.06 哈尔滨兽医研究所猪繁殖与呼吸综合征基础研究组负责人

2008.10-2010.10 美国加州大学旧金山分校博士后

2005.10-2008.10 美国Stowers Institute 博士后

1994.07-1996.09 黄石市农业局

5、承担主要科研项目

1. 国家自然科学基金委地区联合创新基金，非洲猪瘟病毒免疫调节基因的功能及其在病毒感染致病中的作用（260万），2022.1-2025.12，在研，主持。

2. 国家自然科学基金委非洲猪瘟基础研究重点专项，非洲猪瘟感染及致病机制研究（300万），批准号31941002，2019.7-2022.12，在研，主持。

3. 国家自然科学基金委面上基金，免疫球蛋白lambda链及其相似肽抑制I型干扰素产生的分子机制及促进PRRSV复制的研究（15万），批准号31640083，2017.1-2017.12，在研，结题。

4. 中国农业科学院创新工程基金，猪繁殖与呼吸综合征病毒-细菌混合感染基础研究（60万），2016.1-2016.12，结题，主持。

5. 中国农业科学院创新工程基金，猪繁殖与呼吸综合征病毒基础研究（128万），2013.1-2015.12，结题，主持。

6. 国家自然科学基金青年基金，PRRSV NSP1β与HSP40 互作抑制I 型干扰素的产生分子机制（25万），批准号31302163，2014.1-2016.12，结题，参与。

7. 中国农业科学院青年基金配套基金，病毒RNA 感受蛋白DDX19A 功能的研究（10万），批准号302014024，2014.1-2014.12，结题，主持。

8. 中国农业科学院增量项目，重要动物病毒在巨噬细胞中复制及调控天然免疫机制的研究（87万），批准号2013ZL034，2013.1-2015.12，结题，主持。

9. 重点实验室课题，宿主蛋白参与PRRSV 复制机理的研究(15万))，批准号SKLVBP201309，2013.1-2013.12，结题，主持。

10. 国家自然科学基金面上项目，PRRSV 编码蛋白与宿主蛋白相互作用的研究(57万)，批准号31172333，2012.1-2015.12,结题，主持。

11. 重点实验室课题，PRRSV 非结构蛋白NSP1β 抑制天然免疫的分子基础(30万)，批准号SKLVBP2012, 2012.1-2012.12，结题，主持。

12. 重点实验室课题，PRRSV 编码蛋白与宿主蛋白相互作用网络的构建(50万），批准号SKLVBP201026，2011.1-2011.12，结题，主持。

6、代表性文章

1. Guangqiang Ye, Zhaoxia Zhang, Xiaohong Liu, Hongyang Liu, Weiye Chen, Chunying Feng, Jiangnan Li, Qiongqiong Zhou, Dongming Zhao, Shuai Zhang, Hefeng Chen, Zhigao Bu, Li Huang*, Changjiang Weng*, African swine fever virus pH240R enhances viral replication via inhibition of the type I IFN signaling pathway. Journal of Virology. 2024 Feb 14:e0183423.

2. Tingting Li*, Jun Zheng*, Tao Huang, Xiao Wang, Jiangnan Li, Feng Jin, Wenjuan Wei, Xin Chen, Chuanxia Liu, Miaofei Bao, Gaihong Zhao, Li Huang, Dongming Zhao, Jianxin Chen, Zhigao Bu, Changjiang Weng*. African swine fever virus replication inhibitors by screening of a library of FDA-approved drugs. Virology. 2024 Feb 14;593:110014.

3. Guangqiang Ye, Hongyang Liu, Xiaohong Liu, Weiye Chen, Jiangnan Li, Dongming Zhao, Gang Wang, Chunying Feng, Zhaoxia Zhang, Qiongqiong Zhou, Jun Zheng, Zhigao Bu, Changjiang Weng*, Li Huang, African Swine Fever Virus H240R Protein Inhibits the Production of Type I Interferon through Disrupting the Oligomerization of STING. Journal of Virology. 2023; 97(9): e0057723.

4. Li Huanga, Weiye Chena, Hongyang Liua, Mengdi Xuea, Siqi Dong, Xiaohong Liu, Chunying Feng, Shinuo Cao, Guangqiang Ye, Qiongqiong Zhou, Zhaoxia Zhang, Jun Zheng, Jiangnan Li, Dongming Zhao, Zilong Wang, Encheng Sun, Hefeng Chen, Shuai Zhang, Xue Wang, Xianfeng Zhang, Xijun H, Yuntao Guan, Zhigao Bu*, Changjiang Weng*, African Swine Fever Virus HLJ/18 CD2v Suppresses Type I IFN Production and IFN-Stimulated Genes Expression through Negatively Regulating cGMP-AMP Synthase STING and IFN Signaling Pathways. Journal of Immunology. 2023, 210 (9): 1338-1350.

5. Qiongqiong Zhoua, Longfeng Zhanga, Qihong Lin, Hongyang Liu, Guangqiang Ye, Xiaohong Liu, Shuang Jiao, Jiangnan Li, Yandong Tang, Deshi Shi, Li Huang*, Changjiang Weng*, Mechanism of PRV infection activating TLRs-NF-kB axis and AIM2 inflammasome to enhance inflammatory response. Journal of Virology. 2023, 97 (3).

6. Li Huang, Hongyang Liu, Guangqiang Ye, Xiaohong Liu, Weiye Chen, Zilong Wang, Shijun Zhou, Dongming Zhao, Zhaoxia Zhang, Chunying Feng, Xianfeng Zhang, Xijun He, Jun Zheng, Zhigao Bu*, Jiangnan Li*, Changjiang Weng*, ASFV pH240R affects the virulence of African swine fever virus by inhibiting type I interferon signaling and NLRP3-mediated inflammatory responses. Journal of Virology. 2023, 97 (2).

7. Tingting Li*, Xuewen Li*, Xiao Wang, Xin Chen, Gaihong Zhao, Chuanxia Liu, Miaofei Bao, Jie Song, Jiangnan Li, Li Huang, Jun Rong, Kegong Tian, Junhua Deng, Jianzhong Zhu, Xuehui Cai, Zhigao Bu, Jun Zheng, Changjiang Weng*. African swine fever virus pS273R antagonizes stress granule formation by cleaving the nucleating protein G3BP1 to facilitate viral replication. Journal of Biological chemistry, 2023 Jul;299(7):104844.

8. Xin Chen, Jun Zheng, Chuanxia Liu, Tingting Li, Xiao Wang, Xuewen Li, Miaofei Bao, Jiangnan Li, Li Huang, Zhaoxia Zhang, Zhigao Bu, Changjiang Weng*. CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis. Emerg Microbes Infect. 2023 Dec;12(2):2220575.

9. Xin Chen, Jun Zheng, Tingting Li, Chuanxia Liu, Miaofei Bao, Xiao Wang, Xuewen Li, Jiangnan Li, Li Huang, Zhaoxia Zhang, Changjiang Weng*. Coreceptor AXL Facilitates African Swine Fever Virus Entry via Apoptotic Mimicry. Journal of Virology. 2023 Jul 27;97(7):e0061623.

10. Jiangnan Li, Jie Song, Shijun Zhou, Shuai Li, Jia Liu, Tingting Li, Zhaoxia Zhang, Xianfeng Zhang, Xijun He, Weiye Chen, Jun Zheng, Dongming Zhao, Zhigao Bu, Li Huang, Changjiang Weng*. Development of a new effective African swine fever virus vaccine candidate by deletion of the H240R and MGF505-7R genes results in protective immunity against the Eurasia strain. Journal of Virology. 2023 Oct 31;97(10):e0070423.

11. Hongyang Liu1, Guangqiang Ye1, Xiaohong Liu, Mengdi Xue, Qiongqiong Zhou, Longfeng Zhang, Kunli Zhang, Li Huang*, Changjiang Weng*. Vimentin inhibits type I interferon production by disrupting the TBK1-IKKε-IRF3 axis. Cell Reports. 2022, 41, 111469.

12. Xiaohong Liu, Hongyang Liu, Guangqiang Ye, Mengdi Xue, Huibin Yu, Chunying Feng, Qiongqiong Zhou, Xuemin Liu, Longfeng Zhang, Shuang Jiao, Changjiang Weng*, Li Huang*. African swine fever virus pE301R negatively regulates cGAS-STING signaling pathway by inhibiting the nuclear translocation of IRF3. Veterinary Microbiology. 274 (2022) 109556.

13. Qiongqiong Zhou, Longfeng Zhang, Hongyang Liu, Guangqian Ye, Li Huang*, Changjiang Weng*. Isolation and Characterization of Two Pseudorabies Virus and Evaluation of Their Effects on Host Natural Immune Responses and Pathogenicity. Viruses, 2022 14 (4): 712.

14. Li Huang, Jiangnan Li, Jun Zheng, Dan Li, Changjiang Weng*. Multifunctional pMGF505-7R is a key virulence-related factor of African Swine Fever Virus. Frontiers in Microbiology, 2022. 852431.

15. Tingting Li#, Gaihong Zhao#, Taoqin Zhang, Zhaoxia Zhang, Xin Chen, Jie Song, Xiao Wang, Jiangnan Li, Li Huang, Lili Wen, Changyao Li, Dongming Zhao, Xijun He, Zhigao Bu, Jun Zheng*, Changjiang Weng*. African Swine Fever Virus pE199L Induces Mitochondrial-Dependent Apoptosis. Viruses. 2021 Nov 8;13(11):2240.

16. Li Huang#, Wenjie Xu#, Hongyang Liu, Mengdi Xue, Xueming Liu, Kunli Zhang, Liang Hu, Jiangnan Li, Zhida Xiang, Jun Zheng, Changyao Li, Weiye Chen, Zhigao Bu,, Tiao Xiong, Changjiang Weng*. African Swine Fever Virus pI215L Negatively Regulates cGAS-STING Signaling Pathway through Recruiting RNF138 to Inhibit K63-Linked Ubiquitination of TBK1. J Immunol. 2021 Dec 1;207(11):2754-2769.

17. Jiangnan Li#, Jie Song#, Li Kang, Li Huang, Zhijun Zhou, Liang Hu, Jun Zheng, Changyao Li, Zhang X, Xijun He, Dongming Zhao, Zhigao Bu*, Changjiang Weng*. pMGF505-7R determines pathogenicity of African swine fever virus infection by inhibiting IL-1β and type I IFN production. PLoS Pathog. 2021 Jul 26;17(7):e1009733.

19. Li Hu, Xingbo Cheng, Zev Binder, Zhibin Han,Yibo Yin, Donald M O'Rourke, Sida Wang,Yumeng Feng, Changjiang Weng, Anhua Wu, Zhiguo Lin. Molecular and Clinical Characterization of UBE2S in Glioma as a Biomarker for Poor Prognosis and Resistance to Chemo-Radiotherapy. Front Oncol. 2021 May 27;11:640910.

20. Li Du, Honglei Wang, Fang Liu, Zeyu Wei, Changjiang Weng, Jun Tang, Wen-Hai Feng. NSP2 Is Important for Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus to Trigger High Fever-Related COX-2-PGE2 Pathway in Pigs. Front Immunol. 2021 Apr 29;12:657071.

21. Jie Song, Kang Li, Ting Li, Gaihong Zhao, Shijun Zhou, Huang Li, Jiangnan Li, Changjiang Weng*. Screening of PRRSV- and ASFV-encoded proteins involved in the inflammatory response using a porcine iGLuc reporter. J Virol Methods. 2020 Nov;285:113958.

22. Li Huang,# Hongyang Liu, Kunli Zhang, Qingwen Meng, Liang Hu, Yuanfeng Zhang, Zhida Xiang, Jiangnan Li, Yuying Yang, Yali Chen, Shangjin Cui, Hong Tang, Huadong Pei, Zhigao Bu, Changjiang Weng*. Ubiquitin-Conjugating Enzyme 2S Enhances Viral Replication by Inhibiting Type I IFN Production through Recruiting USP15 to Deubiquitinate TBK1. Cell Rep. 2020 Aug 18;32(7):108044.

23. Chao Sun, Mengmeng Liu, Jitao Chang, Decheng Yang, Bo Zhao, Haiwei Wang, Guohui Zhou, Changjiang Weng, Li Yu*. Heterogeneous Nuclear Ribonucleoprotein L Negatively Regulates Foot-and-Mouth Disease Virus Replication through Inhibition of Viral RNA Synthesis by Interacting with the Internal Ribosome Entry Site in the 5' Untranslated Region. J Virol. 2020 May 4; 94(10): e00282-20.

24. Zhaoxia Zhang, Haorong Gu, Qi Li, Jun Zheng, Shinuo Cao, Changjiang Weng, Honglin Jia. GABARAPL2 Is Critical for Growth Restriction of Toxoplasma gondii in HeLa Cells Treated with Gamma Interferon. Infect Immun. 2020 Apr 20; 88(5): e00054-20.

25. Xiaoying Zhi, Yun Zhang, Shiqi Sun, Zhihui Zhang, Hu Dong, Xin Luo, Yanquan Wei, Zengjun Lu, Yongxi Dou, Run Wu, Zhengfan Jiang, Changjiang Weng, Ho Seong Seo, Huichen Guo. NLRP3 inflammasome activation by Foot-and-mouth disease virus infection mainly induced by viral RNA and non-structural protein 2B. RNA Biol. 2020 Mar;17(3):335-349.

26. Kunli Zhang#, Yuanfeng Zhang#, Juan Xue, Qingwen Meng, Hongyang Liu, Caihong Bi, Changyao Li, Liang Hu, Huibin Yu, Tao Xiong, Yuying Yang, Shangjin Cui, Zhigao Bu, Xijun He, Jiangnan Li, Li Huang, Changjiang Weng*. DDX19 Inhibits Type I Interferon Production by Disrupting TBK1-IKKε-IRF3 Interactions and Promoting TBK1 and IKKε Degradation. Cell Rep. 2019 29;26(5):1258-1272.

27. Changyao Li, Yan Chai, Hao Song, Changjiang Weng, Jianxun Qi, Yeping Sun, George F Gao. Crystal Structure of African Swine Fever Virus dUTPase Reveals a Potential Drug Target. MBio. 2019 Oct 29;10(5).

7、申请专利

1. 翁长江、蔡雪辉、李江南、王承宝、刘永刚、黄丽、石文达. 猪繁殖与呼吸综合征病毒疫苗株CH1a cDNA感染性克隆及其应用专利申请号：201310682463.6

2. 翁长江、崔尚金、黄丽、李江南.一种猪脑心肌炎病毒HB10株感染性克隆的构建及应用专利申请号：201410330789.7

3. 基于非洲猪瘟p54蛋白建立ELISA抗体检测试剂盒及应用

4. 基于非洲猪瘟p30蛋白建立ELISA抗体检测试剂盒及应用