Menghang Wang,Ying Yu ,Jianan Wu,Shujie Wang,Luis G Giménez-Lirola,Pablo Piñeyro,Yu Wang,Hongliang Cui,Xijun He,Jeffrey J Zimmerman,Yabin Tu,Xuehui Cai,Gang Wang

Vet Sci.2023 Aug 10;10(8):517.doi: 10.3390/vetsci10080517.

Abstract

Porcine circovirus 3 (PCV3) is an emerging virus first discovered in the United States in 2015, and since then, PCV3 has been found in many regions of the world, including America, Asia, and Europe. Although several PCV3 investigations have been carried out, there is a lack of knowledge regarding the pathogenicity of PCV3, mostly due to the limited number of PCV3 isolates that are readily available. In this study, PCV3-DB-1 was isolated in PK-15 cells and characterized in vitro. Electron microscopy revealed the presence of PCV-like particles, and in situ  hybridization  RNA analysis demonstrated the replication of PCV3 in PK-15 cell culture. Based on phylogenetic analysis of PCV3 isolates from the Heilongjiang province of China, PCV3-DB-1 with 24 alanine and 27 lysine in the Cap protein was originally isolated and determined to belong to the clade PCV3a.

Keywords: PCV-like particles; PCV3 isolation; genomic characteristics; porcine circovirus.