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团队成员

祁小乐

1、个人简历

祁小乐,男,博士,研究员,博士生导师。中国农业科学院哈尔滨兽医研究所禽免疫抑制病创新团队、兽医生物技术国家重点实验室、世界动物卫生组织(WOAH)传染性法氏囊病参考实验室骨干专家。十三五国家重点研发计划首席科学家、全国青年岗位能手、中国农业科学院农科英才;中国兽医协会团体标准技术委员会家禽疾病诊疗分委会秘书长,科技部国家科技专家库专家,教育部学位中心专家,国家自然基金委同行评议专家,黑龙江省农业转基因安全委员会专家组成员;Frontiers in Microbiology等期刊编辑。

主要从事传染性法氏囊病(IBD)等禽免疫抑制病研究,提出了IBDV新的基因分型方法,率先鉴定了IBDV新型变异株新疫情的流行,阐明了我国IBDV的流行新态势;合作解析了迄今分辨率最高的IBDV强、弱毒株粒子结构;率先建立了基于RNA聚合酶II的IBDV高效反向遗传研究平台,揭示了IBDV细胞嗜性、致病和遗传变异的分子机制;创制了首个IBD反向遗传疫苗、首个IBD新型变异株亚单位疫苗等禽免疫抑制病防控制品,积极参加禽免疫抑制病防治新技术的转化推广。主持国家重点研发计划、国家自然科学基金、黑龙江省自然科学基金重点项目等10余项。以第一或通讯作者在Science Bulletin、Antiviral Research、Veterinary Microbiology等期刊发表论文60余篇;参编专著7部;参与制定国家和行业标准4项,获国家发明专利20余项;荣获黑龙江省科技进步一等奖、中华农业科技优秀创新团队一等奖、中华农业科技奖一等奖、大北农科技奖特等奖、中国专利优秀奖等成果奖8项。已培养或合作培养硕、博士研究生20余人(包括哈兽研首批博士留学生),学生多次获得国家奖学金等荣誉,毕业生均在学术单位、政府部门和龙头企业肩负重要职责。

2、联系方式

研究方向:兽医微生物及其分子生物学

电话:0451-51051692(办公室);13845044863(手机)

邮箱:qixiaole@caas.cn

通讯地址:黑龙江省哈尔滨市香坊区哈平路678号

3、教育经历

1997.09-2001.06  河南农业大学 兽医专业 学士

2001.09-2004.06  河南农业大学 预防兽医专业 硕士

2004.09-2007.06  中国农业科学院哈尔滨兽医研究所 预防兽医专业 博士

2012.09   农业部高级专业技术人员理论业务培训班

4、工作经历

2007.07-2009.11  中国农科院哈尔滨兽医研究所 助理研究员

2012.01-2012.04  法国食品环境及劳动卫生署Ploufragan研究所 高级访问学者

2009.12-2017.12  中国农科院哈尔滨兽医研究所 副研究员

2018.01-至今  中国农科院哈尔滨兽医研究所 研究员

5、承担主要项目

[1] 传染性法氏囊病病毒新型变异株免疫逃匿的分子机制(32072852),国家自然科学基金面上项目,2021-2024,58万元,主持;

[2] 鸡传染性法氏囊病病毒细胞嗜性及致病性变异的分子基础(30901083),国家自然科学基金青年基金,2010-2012,20万元,主持;

[3] 禽白血病病毒复制早期关键宿主因子发掘及其作用机制研究(32230105),国家自然科学基金重点项目, 2023-2027,274万元,主要承担人(第2);

[4] 传染性法氏囊病强、弱毒侵染宿主细胞的分子机制(31430087),国家自然科学基金重点项目,2015-2019,318万元,主要承担人(第2);

[5] 家禽主要传染病综合防控研究及国际科技合作平台建设(2016YFE0203200),十三五国家重点研发计划项目,2017-2020,839万元,主持;

[6] 畜禽重要免疫抑制性病原的免疫逃逸机制(2022YFD1800305-1),十四五国家重点研发计划子课题,2022-2025,65万元,主持;

[7] 传染性法氏囊病及马立克氏病等禽免疫抑制病免疫防控新技术研究(2017YFD0500704),十三五国家重点研发计划子课题,2017-2020,53万元,主持;

[8] 中国鸡传染性法氏囊病病毒基因及抗原变异的生物学意义(2011008007),中法蔡元培交流合作项目,2011-2013,17万元,主持;

[9] 鸡传染性法氏囊病病毒超强毒株致弱的主要分子基础(2080440921),中国博士后科学基金,2009-2011,3万元,主持;

[10] 禽双RNA病毒新型变异株遗传变异及免疫逃匿的分子机制(ZD2020C006),黑龙江省自然科学基金重点项目,2020-2023,50万元,主持。

6、代表论文与著作

[1] Niu XX, Han JZ, Huang MM, Wang GD, Zhang YL, Zhang WY, Yu HB, Xu MM, Li K, Gao L, Wang SY, Chen YT, Cui HY, Zhang YP, Liu CJ, Wang XM, Gao YL*, Qi XL*. Infectious bursal disease virus VP5 triggers host shutoff in a transcription-dependent manner. mBio. 2024, 15(3): e0343323.

[2] Jiang N, Wang GD, Zhang WY, Wang YL, Niu XX, Huang MM, Gao L, Li K, Cui HY, Liu CJ, Zhang YP,  Bao KY,  Wang SY, Chen YT, Wang XM, Gao YL*, Qi XL*. A single mutation of VP2 is responsible for the lethality and antigenicity differences between novel variant and very virulent IBDV strains. Transboundary and Emerging Diseases. 2023, 6684304.

[3] Zhang WY, Wang XM, Gao YL*, Qi XL*. The over-40-years-epidemic of infectious bursal disease virus in China. Viruses, 2022, 14(10): 2253.

[4] Bao KY#, Qi XL#, Li Y, Gong MM, Wang XM*, Zhu P*. Cryo-EM structures of infectious bursal disease virus with different virulence provide insights on their assembly and invasion. Science Bulletin, 2022, 67: 646-654.

[5] Fan LJ, Wang YL, Jiang N, Gao YL, Niu XX, Zhang WY, Huang MM, Bao KY, Liu AJ, Wang SY, Gao L, Li K, Cui HY, Pan Q, Liu CJ, Zhang YP, Wang XM, Qi XL*. Residues 318 and 323 in capsid protein are involved in immune circumvention of the epidemic atypical infection of infectious bursal disease virus. Frontiers Microbiology, 2022, 13: 909252.

[6] Jiang N, Wang Y, Zhang W, Niu X, Huang M, Gao Y, Liu A, Gao L, Li K, Pan Q, Liu C, Zhang Y, Cui H, Wang X, Qi X*. Genotyping and molecular characterization of infectious bursal disease virus identified in important poultry-raising areas of China during 2019 and 2020. Frontiers in Veterinary Science, 2021, 8: 759861.

[7] Wang YL, Fan LJ, Jiang N, Gao L, Li K, Gao YL, Liu CJ, Cui HY, Pan Q, Zhang YP, Wang XM*, Qi XL*. An improved scheme for infectious bursal disease virus genotype classification based on both genome-segments A and B. Journal of Integrative Agriculture, 2021, 20(5): 1372-1381.

[8] Wang YL, Jiang N, Fan LJ, Niu XX, Zhang WY, Huang MM, Gao L, Li K, Gao YL, Liu CJ, Cui HY, Liu AJ, Pan Q, Zhang YP, Wang XM, Qi XL*. Identification and pathogenicity evaluation of a novel reassortant infectious bursal disease virus (Genotype A2dB3). Viruses, 2021, 13(9): 1682.

[9] Jiang N, Wang YL, Zhang WY, Niu XX, Gao YL, Gao L, Li K, Cui HY, Liu AJ, Pan Q, Liu CJ, Zhang YP, Wang XM, Qi XL*. Naturally occurring mutated infectious bursal disease virus of genotype A8B1 associated with bursa damage in China. Virus Research, 2021, 302: 198498.

[10] Wang  YL, Jiang N, Fan LJ, Gao L, Li K, Gao YL, Niu XX, Zhang WY, Cui HY, Liu AJ, Pan Q, Liu CJ, Zhang YP, Wang XM*, Qi XL*. Development of a viral‐like particle candidate vaccine against novel variant infectious bursal disease virus. Vaccines, 2021, 9(2):142.

[11]  Fan LJ, Wang YL, Jiang N, Chen MG, Gao L, Li K, Gao YL, Cui HY, Pan Q, Liu CJ, Zhang YP, Wang XM*, Qi XL*. Novel variant infectious bursal disease virus suppresses Newcastle disease vaccination in broiler and layer chickens. Poultry Science, 2020, 99:6542-6548.

[12] Fan LJ, Wang YL, Jiang N, Gao L, Li K, Gao YL, Cui HY, Pan Q, Liu CJ, Zhang YP, Wang XM*, Qi XL*. A reassortment vaccine candidate of the novel variant infectious bursal disease virus. Veterinary Microbiology, 2020, 251: 108905.

[13] Fan LJ, Wu TT, Wang YL, Hussain A, Jiang N, Gao L, Li K, Gao YL, Liu CJ, Cui HY, Pan Q, Zhang YP, Wang XM, Qi XL*. Novel variants of infectious bursal disease virus can severely damage the bursa of fabricius of immunized chickens. Veterinary Microbiology, 2020, 240: 108507.

[14] Wang YL, Fan LJ, Jiang N, Gao L, Li K, Gao YL, Liu CJ, Cui HY , Pan Q, Zhang YP, Wang XM, Qi XL*. Naturally occurring cell-adapted classic strain of infectious bursal disease virus. Veterinary Microbiology, 2020, 243: 108620.

[15] Wu TT, Wang YL, Li H, Fan LJ, Jiang N, Gao L, Li K, Gao YL, Liu CJ, Cui HY, Pan Q, Zhang YP, Wang XM*, Qi XL*. Naturally occurring homologous recombination between novel variant infectious bursal disease virus and intermediate vaccine strain. Veterinary Microbiology, 2020, 245: 108700.

[16] Fan LJ, Wu TT, Hussain A, Gao YL, Zeng XY, Wang YL, Gao L, Li K, Wang YQ, Liu CJ, Cui HY, Pan Q, Zhang YP, Liu YF, He HJ, Wang XM*, Qi XL*. Novel variant strains of infectious bursal disease virus isolated in China. Veterinary Microbiology, 2019, 230: 212-220. (*通讯作者)

[17] Zhang LZ, Li H, Chen YM, Gao X, Lu Z, Gao L, Wang YQ, Gao YL, Gao HL, Liu CJ, Cui HY, Zhang YP, Pan Q, Qi XL*, Wang XM*. The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infection. Virology, 2017, 512: 211-221.

[18] Chen YM, Zhang LZ, Gao L, Wang N, Lu Z, Gao X, Wang YQ, Li K, Gao YL, Cui HY, Gao HL, Liu CJ, Zhang YP, Qi XL*, Wang XM*. Ribosomal protein L4 interacts with viral VP3 and regulates the replication of infectious bursal disease virus. Virus Research, 2016,211: 73-78.

[19] Qi XL, Gao X, Lu Z, Zhang LZ, Wang YQ, Gao L, Gao YL, Li K, Gao HL, Liu CJ, Cui HY, Zhang YP, Wang XM*. A single mutation in the PBC loop of VP2 is involved in the in vitro replication of infectious bursal disease virus. SCIENCE CHINA Life Sciences, 2016(59): 717-723.

[20] Lu Z, Zhang LZ, Wang N, Chen YM, Gao L, Wang YQ, Gao HL, Gao YL, Li K, Qi XL*, Wang XM*. Naturally occurring reassortant infectious bursal disease virus in northern China. Virus Research. 2015, 203: 92-95.

[21] Wang N. Zhang LZ, Chen YM, Lu Z, Gao L, Wang YQ, Gao YL, Gao HL, Cui HY, Li K, Liu CJ, Zhang YP, Qi XL*, Wang XM*. Cyclophilin A interacts with viral VP4 and inhibits the replication of infectious bursal disease virus. BioMed Research International, 2015, 719454.

[22] Zhang LZ, Ren XG, Chen YM, Gao YL, Wang N, Lu Z, Gao L, Qin LT, Wang YQ, Gao HL, Li K, Jiang LL, Cui HY, Liu CJ, Zhang YP, Qi XL*, Wang XM*. Chondroitin sulphate N-acetylgalactosaminyltransferase-2 contributes to the replication of infectious bursal disease virus via interaction with the capsid protein VP2. Viruses, 2015, 7(3): 1474-91.

[23] Deng XY, Hu F, Qi XL*, Gao L, Li K, Gao HL, Gao YL, Wang YQ, Shen N, Hua YP*, Wang XM*. Construction and characterization of the recombinant reticuloendotheliosis virus expressing enhanced green fluorescent protein. Archive Virology, 2015, 160(9): 2231-2235.

[24] Qi XL, Lu Z, Wang N, Chen YM, Zhang LZ, Gao L, Li K, Ren XG, Wang YQ, Gao HL, Gao YL, Nicolas E, Wang XM*. Analysis of the function of D279N mutation of VP2 of infectious bursal disease virus. Journal of Integrative Agriculture, 2015, 14(2): 2618-2625.

[25] Ren XG, Zhang LZ, Gao YL, Gao HL, Wang YQ, Liu CJ, Cui HY, Zhang YP, Jiang LL, Qi XL*, Wang XM*. Binding chicken Anx2 is beneficial for infection with infectious bursal disease virus. Virus Research, 2015,210: 232-240.

[26] Hu F, Zhao Y, Qi XL*, Cui HY, Gao YL, Gao HL, Liu CJ, Wang YQ, Zhang YP, Li K, Wang XM*, Wang YF*. Soluble expression and enzymatic activity evaluation of protease from reticuloendotheliosis virus. Protein Expression and Purification. 2015, 114: 64-70.

[27] Qi XL, Chen YM, Ren XG, Zhang LZ, Gao L, Wang N, Qin LT, Wang YQ, Gao YL, Wang XM*. A reassortment vaccine candidate as the improved formulation to induce protection against very virulent infectious bursal disease virus. Vaccine, 2014, 32: 1436-1443.

[28] Qi XL, Zhang LZ, Chen YM, Gao L, Wu G, Qin LT, Wang YQ, Ren XG, Gao YL, Gao HL, Wang XM*. Mutations of residues 249 and 256 in VP2 are involved in the replication and virulence of infectious bursal disease virus. Plos One, 2013, 8(7):e70982.

[29] Deng XY#, Qi XL#, Wu G, Gao YL, Qin LT, Wang YQ, Gao HL, Wang XM*. Construction and characterization of the infectious clone of reticuloendotheliosis virus carrying a genetic marker. Virus Research, 2012, 167(2):146-151.

[30] Wang YQ#, Qi XL#, Kang ZH, Yu F, Qin LT, Gao HL, Gao YL, Wang XM*. A single amino acid in the C-terminus of VP3 protein influences the replication of attenuated infectious bursal disease virus in vitro and in vivo. Antiviral Research, 2010, 87: 223-229.

[31] Deng XY#, Qi XL#, Gao YL, Wang YQ, Qin LT, Gao HL, Gao L, Wang XM*. Development of a loop-mediated isothermal amplification method for rapid detection of reticuloendotheliosis virus. Journal of Virological Methods, 2010, 168: 82-86. (*共同第1作者)

[32] Qi XL, Gao HL, Gao YL, Qin LT, Wang YQ, Gao L, Wang XM*. Naturally occurring mutations at residues 253 and 284 in VP2 contribute to the cell tropism and virulence of very virulent infectious bursal disease. Antiviral Research, 2009, 84: 225-233.

[33] Qi XL, Gao YL, Gao HL, Deng XY, Bu ZG, Fu CY, Wang XY, Wang XM*. An improved method for infectious bursal disease virus rescue using RNA polymerase II system. Journal of Virological Methods, 2007, 142: 81-88.

[34] 祁小乐 参译,《兽医微生物学》第3版,中国农业出版社,2021.

[35] 祁小乐 参译,《现代马病诊疗学》第7版,中国农业出版社,2020.

[36] 祁小乐 参译,《兽医病毒学》第4版,中国农业出版社,2015.

[37] 祁小乐 参编,《兽医微生物学》第2版,中国农业出版社,2014.

[38] 祁小乐 参编,《动物病毒反向遗传学》,科学出版社,第1版(2009),第2版(2014)

[39] 祁小乐 参编,《动物基因工程疫苗原理与方法》,化学工业出版社,2009.

7、获得主要奖励

[1] 鸡传染性法氏囊病变异的分子机制研究及系列疫苗研发,大北农科技奖特等奖(科技部备案),排名第2.  大北农科技有限公司. 2011.

[2] 禽白血病综合防控关键技术创制与应用,中国农业科学院杰出科技创新奖,排名第3. 中国农业科学院. 2023.

[3] 鸡传染性法氏囊病超强毒的细胞适应株及其应用,中国专利优秀奖,排名第3. 国家知识产权局. 2018年.

[4] 禽白血病防控关键技术的研制与应用,大北农科技奖二等奖(科技部备案),排名第3. 大北农科技有限公司. 2022.

[5] 禽重要免疫抑制病防控关键技术创制与应用,黑龙江省科技进步一等奖,排名第4. 黑龙江省政府. 2018.

[6] 中国农业科学院成果转化优秀团队奖,排名第4. 中国农业科学院. 2022.

[7] 鸡传染性法氏囊病超强毒变异机制及防制技术研究与应用,黑龙江省科技进步一等奖,排名第5. 黑龙江省政府. 2008.

[8] 鸡传染性法氏囊病超强毒变异机制及防制技术研究与应用,中华农业科技奖一等奖,排名第5. 农业部. 2009.

[9] 新型禽用疫苗及诊断试剂研究团队,中华农业科技优秀创新团队奖一等奖,排名第6. 农业部. 2013.

[10] 禽白血病综合防控技术及应用,哈尔滨市科技进步二等奖,排名第9. 哈尔滨市政府. 2016年.

8、主要专利和标准

[1] 祁小乐,王笑梅,高玉龙,高立,崔红玉,李凯,刘长军,潘青,张艳萍.抗IBDV VP2蛋白的单克隆抗体组合及其在鉴别检测IBDV中的用途. 专利授权号:ZL202011466765.6.,中国,2022.12.

[2] 祁小乐,王笑梅,高玉龙,高立,李凯,崔红玉,刘长军,潘青,张艳萍,刘爱晶. 鸡传染性法氏囊病病毒新型变异株亚单位疫苗、其制备方法及应用. 专利授权号:ZL202011473614.3.,中国,2022.12.

[3] 祁小乐,王笑梅,高玉龙,高立,李凯,崔红玉,刘长军,潘青. 一株自然适应体外细胞培养的鸡传染性法氏囊病病毒经典株及其应用,专利授权号:ZL202010090643.5.,中国,2021.8.

[4] 王笑梅,祁小乐,高玉龙,高宏雷,秦立廷,王永强,高立. 鸡传染性法氏囊病病毒重组弱毒疫苗株及其应用,专利授权号:ZL201010215328.7.,中国,2011.12.

[5] 高玉龙,王笑梅,祁小乐,潘青,王永强,刘长军,李凯,高立,王牟平,崔红玉,张艳萍,高宏雷,一种检测A、B亚群禽白血病病毒抗体的ELISA试剂盒及其应用,专利授权号:ZL201910406683.3,中国,2022.2.

[6] 高玉龙,王笑梅,祁小乐,王永强,高宏雷,刘长军,崔红玉,张艳萍,李凯,高立. 检测禽偏肺病毒的RT-PCR的方法,专利授权号:ZL201510599748.2,中国,2018.10.

[7] 国家标准,传染性法氏囊病诊断技术. GB/T19167-2020. 祁小乐参编.

[8] 国家标准,鸡马立克氏病诊断技术. GB/T18643-2021. 祁小乐参编.

[9] 国家行业标准,禽偏肺病毒感染诊断技术,NY∕T4039-2021. 祁小乐参编.

[10] 国家行业标准,I群禽腺病毒检测方法,NY/T4027-2021. 祁小乐参编.



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