1、个人简介

党光辉，男，博士，副研究员，硕士生导师，中国农业科学院哈尔滨兽医研究所动物细菌病创新团队成员，中国防痨协会人兽共患结核病专业分会委员。主持十四五国家重点研发计划子课题、国家自然科学基金等项目3项。以第一（含共第一）和通讯作者发表文章16篇，获得授权发明专利2项。协助培养研究生12名。目前主要从事结核病和副结核病致病机制研究、亚单位疫苗和诊断制品的开发。

2、联系方式

研究方向：兽医微生物及其分子生物学

电话：0451-51051732

邮箱：dangguanghui@caas.cn

通讯地址：黑龙江省哈尔滨市香坊区哈平路678号综合楼5008，150069

3、教育经历

2004.09-2009.06 河南科技学院  动物医学  学士

2010.09-2012.06 福建农林大学  基础兽医学  硕士

2014.09-2018.01 中国农业科学院研究生院  预防兽医学  博士

4、工作经历

2013.07-2014.08 中国农业科学院哈尔滨兽医研究所  科研助理

2018.11-2023.07 中国农业科学院哈尔滨兽医研究所  博士后

2022.01-至今 中国农业科学院哈尔滨兽医研究所  副研究员

5、 承担的项目

1. 国家自然科学基金青年项目，32002256，Rv3194c蛋白酶在结核分枝杆菌逃逸IgG介导的免疫杀伤中的作用机制研究，2021-01-01 至 2023-12-31，24万元， 主持

2. “十四五”国家重点研发计划子课题，2023YFD1801302，“濒危和青藏高原动物结核病和副结核病诊断技术与产品研发”2023-12-01至2027-12-31，60万元，主持

3. 中央级公益性科研院所基本科研业务费项目，1610302024002，XthA磷脂酶影响分枝杆菌分枝菌酸合成的机制研究，2024-01-01至2024-12-31，16万元，主持

4. 国家自然科学基金面上项目，32273005，结核分枝杆菌Rv2569c半胱氨酸蛋白酶介导的Caspase非依赖性巨噬细胞凋亡的机制研究，2023-01-01至2026-12-31，54万元，参与

6、代表论文与著作

1. Zang Xinxin, Zhang Jiajun, Jiang Yanyan, Feng Tingting, Cui Yingying, Wang Hui, Cui Ziyin, Dang Guanghui*, Liu Siguo*. Serine protease Rv2569c facilitates transmission of Mycobacterium tuberculosis via disrupting the epithelial barrier by cleaving E-cadherin. PLoS Pathogens, 2024 20(5): e1012214.

2. Shao Mingzhu, Cui Ning, Tang Yangyang, Chen Fanruo, Cui Yingying, Dang Guanghui*, Liu Siguo*. A candidate subunit vaccine induces protective immunity against Mycobacterium avium subspecies paratuberculosis in mice. NPJ Vaccines. 2023 8(1):72.

3. Zhang Jiajun, Cui Yingying, Zang Xinxin, Feng Tingting, Chen Fanruo, Wang Hui, Dang Guanghui*, Liu Siguo*. PE12 interaction with TLR4 promotes intracellular survival of Mycobacterium tuberculosis by suppressing inflammatory response. International Journal of Biological Macromolecules. 2023 253(Pt 8):127547.

4. Tang Yangyang, Cui Yingying, Jiang Yanyan, Shao Mingzhu, Zang Xinxin, Dang Guanghui*, Liu Siguo*. Characteristics of Mycobacterium tuberculosis serine protease Rv1043c in enzymology and pathogenicity in mice. Journal of Integrative Agriculture. 2023 22(12):3755-3768.

5. Zang Xinxin#, Dang Guanghui#, Cai Zhuming, Shao Mingzhu, Tang Yangyang, Cao Jun, Cui Ziyin, Liu Siguo*. (2022). Extracellular DNase MAP3916c attacks the neutrophil extracellular traps and is needed for Mycobacterium avium subsp. paratuberculosis virulence. Veterinary microbiology. 2022, 273, 109529.

6. Cui Yingying#, Tang Yangyang#, Shao Mingzhu, Zang Xinxin, Jiang Yanyan, Cui Ziyin, Dang Guanghui*, Liu Siguo*. Mycobacterium tuberculosis protease Rv3090 is associated with late cell apoptosis and participates in organ injuries and mycobacterial dissemination in mice. Microbial pathogenesis, 2022, 173(Pt B), 105880.

7. Liu Hongxiu#, Dang Guanghui#, Zang Xinxin, Cai Zhuming, Song Ningning, Liu Siguo*. Characterization and pathogenicity of extracellular serine protease MAP3292c from Mycobacterium avium subsp. paratuberculosis. Microbial Pathogenesis. 2020, 142: 104055.

8. Li He#, Dang Guanghui#, Liu Hongxiu, Wang Zhongxing, Cui Ziyin, Song Ningning, Chen Liping, Liu Siguo. Characterization of a novel Mycobacterium tuberculosis serine protease (Rv3194c) activity and pathogenicity. Tuberculosis. 2019, 119:101880.

9. Dang Guanghui, Cui Yingying, Wang Lei, Li Tiantian, Cui Ziyin, Song Ningning, Chen Liping, Pang Hai, Liu Siguo*. Extracellular Sphingomyelinase Rv0888 of Mycobacterium tuberculosis Contributes to Pathological Lung Injury of Mycobacterium smegmatis in Mice via Inducing Formation of Neutrophil Extracellular Traps. Frontiers in immunology. 2018, 9:677.

10. Dang Guanghui, Cao Jun, Cui Yingying, Song Ningning, Chen Liping*, Pang Hai*, Liu Siguo*. Characterization of Rv0888, a Novel Extracellular Nuclease from Mycobacterium tuberculosis. Scientific Reports. 2016, 6:19033.

11. Dang Guanghui#, Chen Liping#, Li Zhaoli, Deng Xiaoxia, Cui Yingying, Cao Jun, Yu Shenye, Pang Hai*, Liu Siguo*. Expression, Purification and Characterisation of Secreted Esterase Rv2525c from Mycobacterium tuberculosis. Applied Biochemistry and Biotechnology. 2015, 176(1): 1-12.

12. Chen Liping#, Dang Guanghui#, Deng Xiaoxia, Cao Jun, Yu Shenye, Wu Defeng, Pang Hai*, Liu Siguo*. Characterization of a novel exported esterase Rv3036c from Mycobacterium tuberculosis. Protein Expression and Purification. 2014, 104: 50-56.

13. 陈凡若, 张嘉俊, 鹿萍, 崔宁, 崔莹莹, 崔子寅, 党光辉*, 刘思国*. 副结核分枝杆菌免疫原蛋白的筛选及免疫保护效果评价[J]. 中国农业科学, 2024, 57 (06): 1204-1214.

14. 蔡珠明#, 党光辉#, 臧鑫鑫, 邵明珠, 唐阳阳, 鹿萍, 崔莹莹, 崔子寅, 刘思国. 结核分枝杆菌复合群、副结核分枝杆菌和禽分枝杆菌禽亚种多重TaqMan荧光定量PCR方法的建立及初步应用[J]. 中国预防兽医学报, 2023, 45 (01): 51-59.

15. 陈凡若#, 党光辉#, 张嘉俊, 鹿萍, 崔宁, 崔莹莹, 崔子寅, 刘思国. 结核分枝杆菌PE26蛋白调节巨噬细胞免疫反应进而促进分枝杆菌胞内定殖[J]. 中国预防兽医学报, 2023, 45 (05): 502-509.

16. 臧鑫鑫#，党光辉#，王忠星，刘虹秀，崔子寅，崔莹莹，宋宁宁，刘思国. 耻垢分支杆菌能够诱导中性粒细胞胞外诱捕网的形成. 中国预防兽医学报. 2019, 41(11): 1154-1158.

7、专利

1. 党光辉，刘思国，蔡珠明，崔子寅. 一种同时检测三种分枝杆菌的多重TaqMan荧光定量PCR试剂盒及其应用. 专利号：ZL202110731289.4

2. 刘思国，党光辉，陈利苹，李田田. 一种牛副结核间接ELISA检测试剂盒及其应用. 专利号：ZL202310215284.5