1、个人简历

谢芳，女，博士，副研究员，硕士生导师，动物细菌病研究创新团队科研骨干。研究领域为病原菌与宿主互作、抗菌药物及其机制研究，先后主持和参与国家自然科学基金、黑龙江省科学基金等课题10余项，以第1作者或通讯作者在NPJ Biofilms Microbi, J Biol Chem, Int J Mol Sci等杂志发表SCI文章10余篇；获得国家发明专利10余项，获得省市级奖励4项；参编著作1部。

2、联系方式    

研究方向：兽医微生物及其分子生物学

电话：0451-51051731

邮箱：xiefang@caas.cn

所在单位：中国农业科学院哈尔滨兽医研究所

通讯地址：哈尔滨市香坊区哈平路678号哈尔滨兽医研究所，150069

3、教育经历

2001.09-2005.06  河北科技师范学院  动物医学专业  学士

2005.09-2007.06  吉林大学  预防兽医学专业  硕士

2007.09-2010.06  吉林大学  预防兽医学专业  博士

4、工作经历

2010.07-2017.12  中国农科院哈尔滨兽医研究所  助理研究员

2018.01-至今  中国农科院哈尔滨兽医研究所  副研究员

5、主持项目

1. 国家自然科学基金面上项目：猪链球菌2型半胱氨酸蛋白酶ApdS切割宿主防御肽cathelicidin LL-37介导其免疫逃逸的分子机制（31873016, 2019.01-2022.12）, 61万元；

2. 国家自然科学基金青年科学基金项目：猪胸膜肺炎放线杆菌ClpP蛋白酶的致病机理研究（31100103, 2012.01-2014.12），24万元；

3. 黑龙江省科学基金青年项目：猪链球菌2型SSU05_1311蛋白与纤连蛋白互作介导其黏附和侵入宿主细胞的分子机制（QC2016044, 2016.07-2019.07），5万元；

4. 中国农业科学院基本科研业务费专项：甲氧西林耐药金黄色葡萄球菌抗菌新药创制（1610302017007, 2017.01-2020.12）, 244万元。

6、代表论文与著作

1. Huihui Zhang #, Xinyuan Zhang, Siyu Liang, Jing Wang, Yao Zhu, Wanjiang Zhang, Siguo Liu, Stefan Schwarz*, Fang Xie*. Bactericidal synergism between phage endolysin Ply2660 and cathelicidin LL-37 against vancomycin-resistant Enterococcus faecalis biofilms. NPJ Biofilms and Microbiomes. 2023, 9(1):16.

2. Mingjie Jin, Siyu Liang, Jing Wang, Huihui Zhang, Yueling Zhang, Wanjiang Zhang, Siguo Liu*, Fang Xie *. Endopeptidase O promotes Streptococcus suis immune evasion by cleaving the host-defence peptide cathelicidins, Virulence, 2023, 14(1):2283896.

3. Jing Wang, Siyu Liang, Xiaofeng Lu, Qiu Xu, Yao Zhu, Shenye Yu, Wanjiang Zhang, Siguo Liu*, Fang Xie *. Bacteriophage endolysin Ply113 as a potent antibacterial agent against polymicrobial biofilms formed by enterococci and Staphylococcus aureus. Frontiers in Microbiology. 2023,14:1304932.

4. Fang Xie, Yanan Zan, Xinyuan Zhang, Huihui Zhang, Mingjie Jin, Wanjiang Zhang, Yueling Zhang, Siguo Liu*. Differential abilities of mammalian cathelicidins to inhibit bacterial biofilm formation and promote multifaceted immune functions of neutrophils. International Journal of Molecular Sciences. 2020, 21(5):1871.

5. Fang Xie, Yanan Zan, Yueling Zhang, Ning Zheng, Qiulong Yan, Wanjiang Zhang, Huihui Zhang, Mingjie Jin, Fuguang Chen, Xinyuan Zhang, Siguo Liu*. The cysteine protease ApdS from Streptococcus suis promotes evasion of innate immune defenses by cleaving the antimicrobial peptide cathelicidin LL-37. Journal of Biological Chemistry. 2019, 294(47): 17962-17977.

6. Fang Xie, Yalei Wang, Gang Li, Shuanghong Liu, Ning Cui, Siguo Liu, Paul R. Langford and Chunlai Wang. The SapA protein is involved in resistance to antimicrobial peptide PR-39 and virulence of Actinobacillus pleuropneumoniae. Frontiers in Microbiology. 2017, 8: 811.

7. Fang Xie, Gang Li, Yalei Wang, Yanhe Zhang, Long Zhou, Chengcheng Wang, Shuanghong Liu, Siguo Liu, Chunlai Wang. Pyridoxal phosphate synthases PdxS/PdxT are required for Actinobacillus pleuropneumoniae viability, stress tolerance and virulence. PLoS One. 2017, 12(4): e0176374.

8. Fang Xie, Gang Li, Long Zhou, Yanhe Zhang, Ning Cui, Siguo Liu, Chunlai Wang. Attenuated Actinobacillus pleuropneumoniae double-deletion mutant S-8∆clpP/apxIIC confers protection against homologous or heterologous strain challenge. BMC Veterinary Research. 2017, 13(1): 14.

9. Gang Li#, Fang Xie#, Jianjun Li, Jiao Liu, Dapeng Li, Yanhe Zhang, Paul R. Langfordb, Yanwen Li, Siguo Liu, Chunlai Wang. Identification of novel Haemophilus parasuis serovar 5 vaccine candidates using an immunoproteomic approach. Journal of Proteomics.2017, 163: 111-117.

10. Fang Xie, Gang Li, Wanjiang Zhang, Yanhe Zhang, Long Zhou, Shuanghong Liu, Siguo Liu, Chunlai Wang. Outer membrane lipoprotein VacJ is required for the membrane integrity, serum resistance and biofilm formation of Actinobacillus pleuropneumoniae. Veterinary Microbiology. 2016, 183: 1-8,

11. Fang Xie, Gang Li, Yanhe Zhang, Long Zhou, Shuanghong Liu, Siguo Liu, Chunlai Wang. The Lon protease homologue LonA, not LonC, contributes to the stress tolerance and biofilm formation of Actinobacillus pleuropneumoniae. Microbial Pathogenesis. 2016, 93: 38-43.

12. Fang Xie, Yanhe Zhang, Gang Li, Siguo Liu, Chunlai Wang. The ClpP protease is required for the stress tolerance and biofilm formation in Actinobacillus pleuropneumoniae. PLoS One. 2013, 8(1): e53600.

13. FengYang#, Fang Xie#, Yuwen Jiang, Suqing Li, Yanping Xing, Yu Wang, Xin Feng, Wenyu Han, Liancheng Lei. Genomic Differences Between Actinobacillus pleuropneumoniae Serotypes 5b and 3 and their Distribution and Transcription Among 15 Serotypes. Current Microbiology. 2011, 63(4): 327-331.

14. Fang Xie, Liancheng Lei, Chongtao Du, Shuqing Li, Wenyu Han, Zhiyan Ren. Genomic differences between Actinobacillus pleuropneumoniae serotypes 1 and 3 and the diversity distribution among 15 serotypes. FEMS Microbiology Letters. 2010, 303(2): 147-155.

15. Fang Xie, Mingjun Zhang, Shuqing Li, Chongtao Du, Changjiang Sun, Wenyu Han, Liang Zhou, Liancheng Lei. Differentially expressed genes in the pathogenic strains of Actinobacillus pleuropneumoniae serotypes 1 and 3. Journal of Microbiology and Biotechnology. 2010, 20(4): 789-797.

16. Fuguang Chen, Fang Xie, Baoling Yang, Chengcheng Wang, Siguo Liu, Yueling Zhang. Streptococcus suis sortase A is Ca2+ independent and is inhibited by acteoside, isoquercitrin and baicalin. PLoS One. 2017, 12(3): e0173767.

17. Gang Li, Fang Xie, Yanhe Zhang, Janine T. Bossé, Paul R. Langford, and Chunlai Wang. Role of (p)ppGpp in viability and biofilm formation of Actinobacillus pleuropneumoniae S8. PLoS One. 2015, 10(10): e0141501.

18. Gang Li, Fang Xie, Yanhe Zhang, Chunlai Wang, Draft genome sequence of Actinobacillus pleuropneumoniae serotype 7 strain S-8. Journal of Bacteriology. 2012, 194(23): 6606-6607.

19. Mingming Hu, Yanhe Zhang, Fang Xie, Gang Li, Jianjun Li, Wei Si, Siguo Liu, Shouping Hu, Zhuo Zhang, Nan Shen, Chunlai Wang. Protection of piglets by a Haemophilus parasuis ghost vaccine against homologous challenge. Clin Vaccine Immunol, 2013, 20(6):795-802.

7、专利和标准

1. 谢芳，刘思国，张万江，张跃灵，一种广谱型抗菌肽及其应用，ZL 202010037156.2

2. 谢芳，王春来，张艳禾，李建军，朱晓凯，李艳玲， 一种不含抗性标记的猪胸膜肺炎放线杆菌ClpP蛋白酶基因缺失株、其构建方法及应用，ZL 201110336861.3

3. 王春来，谢芳，李刚，张艳禾，一种不含抗性标记的猪传染性胸膜肺炎弱毒活疫苗及其用途，ZL 201310469680.7

4. 王春来，谢芳，张艳禾，李刚，一种不含抗性标记的猪胸膜肺炎放线杆菌双基因缺失株、其构建方法及应用，ZL 201110420236.7

5. 王春来，李刚，张艳禾，谢芳，一种副猪嗜血杆菌病亚单位疫苗组合物及应用，ZL 201310406551.3

6. 王春来，李刚，张艳禾，谢芳，一种制备副猪嗜血杆菌病菌影疫苗的方法及其产品和应用，ZL 201310409237.0

7. 王春来，李刚，张艳禾，谢芳， 一种副猪嗜血杆菌免疫保护性抗原AfuA，ZL 20131 0268347.X

8. 李刚，王春来，张艳禾，谢芳，一种副猪嗜血杆菌免疫保护性抗原HbpA，ZL 20131 0268864.7

9. 王春来，李刚，张艳禾，谢芳，一种副猪嗜血杆菌免疫保护性抗原OppA，ZL 20131 0268863.2

8、获得奖励

1. 猪传染性胸膜肺炎综合防控技术研究，中国商业联合会科学技术三等奖，雷连成、李娜，王春来，谢芳，王磊等，2023年

2. 猪传染性胸膜肺炎菌影疫苗的研制及其应用，黑龙江省科技进步三等奖，王春来，李刚，谢芳，张艳禾，李艳玲，周泷，2017年
   

3. 猪传染性胸膜肺炎菌影疫苗的研制及其应用，哈尔滨市科技进步二等奖，王春来，李刚，谢芳，张艳禾，李艳玲，周泷，2016年

4. 基于比较基因组学的胸膜肺炎放线杆菌黏附致病机制及异源免疫研究，吉林省自然科学学术成果奖二等奖，雷连成，冯新，杨舒心，谢芳，王瑜，杜崇涛，孙长江，翟瑞东，李林溪，王磊，杨鹏，周靓，韩文瑜，2014年