1、个人简历

刘思国，男，博士，研究员，博士生导师。中国农业科学院三级岗位科学家，中国防痨协会人兽共患结核病专业分会副主任委员、农业部第七届兽药评审专家。研究方向为动物结核病和副结核病致病机理及防控技术研究。承担了973项目、863项目、重点研发计划和国家自然科学基金项目等20余项课题。发表SCI收录文章100余篇，其中以第1或通讯作者发表76篇。著作8部，获省部级奖6项，获发明专利24项。

2、联系方式

研究方向：动物传染病病原学与流行病学

电话：0451-51051737

邮箱：liusiguo@caas.cn

通讯地址：黑龙江省哈尔滨市香坊区哈平路678号，邮编150069。

3、教育经历

1987.09-1991.06 吉林农业大学 兽医公共卫生专业 学士

1991.09-1994.06 吉林农业大学 兽医微生物和免疫学专业 硕士

1996.09-1999.06 东北农业大学 兽医病理学专业 博士

4、工作经历

2009.1-至今 中国农业科学院哈尔滨兽医研究所 主任/研究员

2001.12-2008.12  中国农业科学院哈尔滨兽医研究所 副研究员

1994.07-2001.12  中国农业科学院哈尔滨兽医研究所 助理研究员

1999.12-2001.12  中国人民解放军军需大学军事兽医研究所 博士后

5、承担项目

1. 十四五国家重点研发计划项目：胞内菌入侵与持续感染机制研究，2021.06.01-2025.12.31，172万元，刘思国主持（子课题）；

2. 国家自然科学基金面上项目：结核分枝杆菌Rv2569c半胱氨酸蛋白酶介导的Caspase非依赖性巨噬细胞凋亡的机制研究（32273005, 2023-2026）,54万元；

3. 国家自然科学基金面上项目：结核分枝杆菌胞外鞘磷脂酶Rv0888诱导肺脏中性粒细胞胞外诱捕网形成继而促进炎症反应的机制研究（31772767，2018.01-2021.12），58万元；

4. 农业部行业专项：东北地区畜禽沙门菌耐药性分析及防控技术研究（201403054，2014-2018），110万元；

5. 农业部行业专项，猪传染性胸膜肺炎放线杆菌的病原分离鉴定与流行病学调查研究（201303034，2013-2017），123万元；

6、代表论文与著作

1. Zang X, Zhang J, Jiang Y, Feng T, Cui Y, Wang H, Cui Z, Dang G*, Liu S*. Serine protease Rv2569c facilitates transmission of Mycobacterium tuberculosis via disrupting the epithelial barrier by cleaving E-cadherin. PLoS Pathog. 2024 May 9;20(5):e1012214.

2. Zhang J, Cui Y, Zang X, Feng T, Chen F, Wang H, Dang G, Liu S. PE12 interaction with TLR4 promotes intracellular survival of Mycobacterium tuberculosis by suppressing inflammatory response. Int J Biol Macromol. 2023 Dec 31;253(Pt 8):127547.

3. Tang Yang#, Cui Y#, Jiang Yan, Shao M, Zang Xin, Dang G*, Liu S*. Characteristics of Mycobacterium tuberculosis serine protease Rv1043c in enzymology and pathogenicity in mice. J Integr Agric. 2023 22(12):3755-3768.

4. Yang Qin, Zhu Yao, Schwarz Stefan, Wang Lingli, Yang Wenlin, Liu Siguo*, Zhang Wanjiang*. Integrative and conjugative elements in streptococci can act as vectors for plasmids and translocatable units integrated via IS1216E. International Journal of Antimicrobial Agents. 2023 Mar 16:106793.

5. Shao M, Cui N, Tang Y, Chen F, Cui Y, Dang G*, Liu S*. A candidate subunit vaccine induces protective immunity against Mycobacterium avium subspecies paratuberculosis in mice. NPJ Vaccines. 2023 May 20;8(1):72.

6. Yue Jia, Juzhao Liu, Qin Yang, Wanjiang Zhang, Thomas Efferth, Siguo Liu*, Xin Hua*. Cajanin stilbene acid：A direct inhibitor of MCR-1 that restores the efficacy of polymyxin B against resistant Gram-negative bacteria. Phytomedicine. 2023,114:154803.

7. Cui Y, Tang Y, Shao M, Zang X, Jiang Y, Cui Z, Dang G*, Liu S*. Mycobacterium tuberculosis protease Rv3090 is associated with late cell apoptosis and participates in organ injuries and mycobacterial dissemination in mice. Microb Pathog. 2022 Dec;173(Pt B):105880.

8. Zang X#, Dang G#, Cai Z, Shao M, Tang Y, Cao J, Cui Z, Liu S*. Extracellular DNase MAP3916c attacks the neutrophil extracellular traps and is needed for Mycobacterium avium subsp. paratuberculosis virulence. Vet Microbiol. 2022 Oct;273:109529.

9. Cui Y, Dang G, Wang H, Tang Y, Lv M, Zang X, Cai Z, Cui Z, Cao J, Liu S*, Song N*. DosR Regulates the Transcription of the Arginine Biosynthesis Gene Cluster by Binding to the Regulatory Sequences in Mycobacterium bovis Bacille Calmette-Guerin. DNA Cell Biol. 2022 Dec;41(12):1063-1074.

10. Cui Z, Dang G, Song N, Cui Y, Li Z, Zang X, Liu H, Wang Z, Liu S*. Rv3091, an extracellular patatin-like phospholipase in Mycobacterium tuberculosis, prolongs intracellular survival of recombinan Mycolicibacterium smegmatis by mediating phagosomal escape. Front Microbiol, 2020, 11:2204.

11. Xie F, Zan Y, Zhang X, Zhang H, Jin M, Zhang W, Zhang Y, Liu S. Differential abilities of mammalian cathelicidins to inhibit bacterial biofilm formation and promote multifaceted immune functions of neutrophils. Int J Mol Sci, 2020, 21(5):1871.

12. Song N, Zhu Y, Cui Y, Lv M, Tang Y, Cui Z, Dang G, Zheng H, Liu S. Vitamin B and vitamin C affect DNA methylation and amino acid metabolism in Mycobacterium bovis BCG. Front Microbiol, 2020, 11:812.

13. Zhu Y, Zhang W, Wang C, Liu W, Yang Q, Luan T, Wang L, Schwarz S, Liu S. Identification of a novel optrA-harbouring transposon, Tn6823, in Staphylococcus aureus. J Antimicrob Chemother, 2020, 75(11):3395-3397.

14. Liu H, Dang G, Zang X, Cai Z, Cui Z, Song N, Liu S. Characterization and pathogenicity of extracellular serine protease MAP3292c from Mycobacterium avium subsp. paratuberculosis. Microb Pathog, 2020, 142:104055.

15. Li G, Zhao Q, Luan T, Hu Y, Zhang Y, Li T, Wang C, Xie F, Zhang W, Langford PR, Liu S. Basal-level effects of (p)ppGpp in the absence of branched-chain amino acids in Actinobacillus pleuropneumoniae. J Bacteriol, 2020, 202(8):e00640-19.

16. Xie F, Zan Y, Zhang Y, Zheng N, Yan Q, Zhang W, Zhang H, Jin M, Chen F, Zhang X, Liu S. The cysteine protease ApdS from Streptococcus suis promotes evasion of innate immune defenses by cleaving the antimicrobial peptide cathelicidin LL-37. J Biol Chem, 2019, 294(47):17962-17977.

17. Wang C, Li P, Yan Q, Chen L, Li T, Zhang W, Li H, Chen C, Han X, Zhang S, Xu M, Li B, Zhang X, Ni H, Ma Y, Dong B, Li S, Liu S. Characterization of the pig gut microbiome and antibiotic resistome in industrialized feedlots in China. mSystems, 2019, 4(6): e00206-19.

18. Zhang W, Zhu Y, Wang C, Liu W, Li R, Chen F, Luan T, Zhang Y, Schwarz S, Liu S. Characterization of a multidrug-resistant porcine Klebsiella pneumoniae sequence type 11 strain coharboring blaKPC-2 and fosA3 on two novel hybrid plasmids. mSphere, 2019, 4(5):e00590-19.

19. Hua X, Jia Y, Yang Q, Zhang W, Dong Z, Liu S. Transcriptional analysis of the effects of gambogic acid and neogambogic acid on methicillin-resistant Staphylococcus aureus. Front Pharmacol, 2019, 10:986.

20. Chen F, Zhang W, Schwarz S, Zhu Y, Li R, Hua X, Liu S. Genetic characterization of an MDR/virulence genomic element carrying two T6SS gene clusters in a clinical Klebsiella pneumoniae isolate of swine origin. J Antimicrob Chemother, 2019, 74(6):1539-1544.

21. Zhu Y, Zhang W, Schwarz S, Wang C, Liu W, Chen F, Luan T, Liu S. Characterization of a blaIMP-4-carrying plasmid from Enterobacter cloacae of swine origin. J Antimicrob Chemother, 2019, 74(7):1799-180.

22. Li H, Dang G, Liu H, Wang Z, Cui Z, Song N, Chen L, Liu S. Characterization of a novel Mycobacterium tuberculosis serine protease (Rv3194c) activity and pathogenicity. Tuberculosis, 2019, 119:101880.

23. Song N, Li Z, Cui Z, Chen L, Cui Y, Dang G, Li Z, Li H, Liu S. The prominent alteration in transcriptome and metabolome of Mycobacterium bovis BCG str. Tokyo 172 induced by vitamin B1. BMC Microbiol, 2019, 19(1):104.

24. Dang G, Cui Y, Wang L, Li T, Cui Z, Song N, Chen L, Pang H, Liu S. Extracellular sphingomyelinase Rv0888 of Mycobacterium tuberculosis contributes to pathological lung injury of Mycobacterium smegmatis in mice via inducing formation of neutrophil extracellular traps. Front Immunol, 2018, 9:677.

25. Hua X, Yang Q, Zhang W, Dong Z, Yu S, Schwarz S, Liu S. Antibacterial activity and mechanism of action of aspidinol against multi-drug-resistant methicillin-resistant Staphylococcus aureus. Front Pharmacol, 2018, 9:619.

26. Wei Si, Shenye Yu, Henggui Liu, Chunlai Wang, Liping Chen, Gaoling Wang, Jianan Wu, Siguo Liu*. A bacterial ghost improves the immunological efficacy of a Newcastle disease virus inactivated vaccine. Vet Microbiol, 2017, 203:189-195.

27. Xiu-Mei Wang, Zhimin Dong, Stefan Schwarz, Zhu Yao, Xin Hua, Yanhe Zhang, Siguo Liu* and Wan-Jiang Zhang*. Plasmids of diverse Inc groups disseminate the fosfomycin resistance gene fosA3 among Escherichia coli isolates from pigs, chickens, and dairy cows in northeast China. Antimicrob Agents Chemother, 2017, 61(9): e00859.

28. Guanghui Dang, Jun Cao, Yingying Cui, Ningning Song, Liping Chen*, Hai Pang*, Siguo Liu*. Characterization of Rv0888, a novel extracellular nuclease from Mycobacterium tuberculosis. Sci Rep, 2016, 6:10933.

29. Wan-Jiang Zhang, Xiu-Mei Wang, Lei Dai, Xin Hua, Zhimin Dong, Stefan Schwarz, and Siguo Liu*. Novel conjugative plasmid from Escherichia coli of swine origin that coharbors the multiresistance gene cfr and the extended-spectrum-β-lactamase gene blaCTX-M-14b. Antimicrob Agents Chemother. 2015, 59(2):1337-1340.

30. Hongbin Si, Wan-Jiang Zhang, Shengbo Chu, Xiu-Mei Wang, Lei Dai, Xin Hua, Zhimin Dong, Stefan Schwarz, and Siguo Liu*, Novel plasmid-borne multidrug resistance gene cluster including lsa(E) from a linezolid-resistant Enterococcus faecium isolate of swine origin. Antimicrob Agents Chemother, 2015, 59(11), 7113-7116.

31. Jun Cao, Guanghui Dang, Huafang Li, Tiantian Li, Zhiguo Yue, Na Li, Yajun Liu, Siguo Liu*, Liping Chen*. Identification and characterization of lipase activity and immunogenicity of LipL from Mycobacterium tuberculosis. PLoS One, 2015, 10(9): e0138151.

32. Wan-Jiang Zhang, Xing-Ran Xu, Stefan Schwarz, Xiu-Mei Wang, Lei Dai, Hua-Jun Zheng and Siguo Liu*. Chracterization of the IncA/C plasmid pSCEC2 harboring the multiresistance cfr gene from Escherichia coli of swine origin. J Antimicrob Chemother, 2014, 69(2):385-389.

33. XiuMei Wang, Xing Feng Lu, Lu Yin, HuiFang Liu, WanJiang Zhang, Wei Si, ShenYe Yu, Meili Shao*, Siguo Liu*. Occurrence and antimicrobial susceptibility of Listeria Monocytogens isolate from retail raw foods. Food Control, 2013, 32: 153-158.

34. Xianglian Yuan*, Liping Chen*, Jun Cao, Qiulong Yan, Yinbin Liu, Guanghui Dang, Shenye Yu, Wei Si, Xiumei Wang, Hai Pang*, Siguo Liu*. Characterization of Rv0394c gene encoding hyaluronidase and chondrosulfatase from Mycobacterium tuberculosis. Tuberculosis, 2013, 93:396-300.

35. Wan-Jiang Zhang, Zongji Lu, Stefan Schwarz, Rong min Zhang, ShenYe Yu, Wei Si, LiPing Chen, Siguo Liu*. Complete sequence of the blaNDM-1-carrying plasmid pNDM-AB from Acinetobacter baumannii of food animal origin. 2013, J Antimicrob Chemother, 2013, 68(7): 1681-1682.

36. Wang XM, Zhang WJ, Schwarz S, Yu SY, Liu H, Si W, Zhang RM, Siguo Liu*. Methicillin-resistant Staphylococcus aureus ST9 from a case of bovine mastitis carries the genes cfr and erm(A) on a small plasmid. J Antimicrob Chemother, 2012, 67(5):1287-1289.

37. Wei Peng§, Lu Yin§, Wei Si§, Huifang Liu, Shenye Yu, Siguo Liu*, Chunlai Wang, Yuehong Chang, Zhuo Zhang, Shouping Hu, Yanfen Du. Salmonella enteritidis ghost vaccine induces effective protection against lethal challenge in specific-pathogen-free chicks. Immunobiology, 2011, 216:558-565.

38. Jiubiao Guo, Lipeng Xu, Xiangdong Zeng, shentao Li, Xianggang Kong, Siguo Liu* and Hai Pang*. Crystal structure of a novel esterase Rv0045c from Mycobacterium tuberculosis. PLoS One, 2011, 6(5) e20506.

39. JiubiaoGuo, Xiangdong Zheng, Lipeng Xu, Zhongyuan Liu, Kehui Xu, Shentao Li, Tingyi Wen, Siguo Liu* and Hai Pang*. Characterization of a novel esterase Rv0045c from Mycobacterium tuberculosis, PLoS One, 2010, 5(10): e13143.

40. Si-Guo Liu, She-Ping Guo, Chun-Lai Wang, Mei-Li Shao, Xiu-Hua Zhang, Yang-Guo, Qiang-Gong. A novel fusion protein based indirect enzyme-linked immunosorbent assay for the detection of bovine tuberculosis. Tuberculosis, 2007, 3: 212-217.

7、专利和标准

1. 党光辉，刘思国，蔡珠明，崔子寅. 一种同时检测三种分枝杆菌的多重TaqMan荧光定量PCR试剂盒及其应用. 专利号：ZL202110731289.4

2. 刘思国，党光辉，陈利苹，李田田. 一种牛副结核间接ELISA检测试剂盒及其应用. 专利号：ZL202310215284.5

3. 于申业，刘思国. 抗大肠杆菌感染的治疗性单克隆抗体、产生该单克隆抗体的杂交瘤细胞株及其用途（ZL201410412146.7），2017年；

4. 于申业，刘思国. 抗phiX174噬菌体裂解蛋白E的单克隆抗体、产生该抗体的杂交瘤细胞株及其表位（ZL201210001050.2），2013年；

5. 刘思国，黎观红，贾永杰，洪智敏，司微，于申业. 一种乳酸杆菌细胞壁完整肽聚糖及其在提高IEC抗菌肽表达水平中的应用（ZL201210122033.4），2015年；

6. 刘思国，王华南，于申业，朱婷，于秀婷，刘慧芳，王秀梅，司微. 抗结核分枝杆菌单克隆抗体及其识别的抗原表位和用途（ZL201110269165.5），2013年；

7. 刘思国，刘慧芳，王秀梅，于申业，王牟平，司微，杨志. 减毒沙门氏菌及其应用（ZL201110061628.9），2012年；

8. 刘思国，常月红，彭伟，刘慧芳，王春来，司微. 重排噬菌体溶菌基因及其打孔载体和在制备疫苗中的应用（ZL200910118379.5），2011年；

9. 刘思国，彭伟，常月红，刘慧芳，王春来，司微. 增强噬菌体溶菌基因打孔效率的DNA序列及其应用（ZL200910118380.8），2011年；

10. 刘思国，郭设平，王春来，张秀华，郭洋，邵美丽.一种诊断牛结核病的重组抗原蛋白及其制备方法（ZL200510005002.0），2008年；

8、获得奖励

1. 人兽共患性结核病新型诊断技术及其在牛结核控制和净化中的应用，湖北省技术发明二等奖，郭爱珍、陈颖钰、刘思国、赵洪进、但汉并、李雪平（华中农业大学、中国农业科学院哈尔滨兽医研究所、上海市动物疫病预防控制中心、武汉科前生物股份有限公司、广州悦洋生物技术有限公司），2020年；

2. 人畜间结核病流行病学的调查研究及推广应用，中华人民共和国农业部农牧渔业丰收奖三等奖，李爱巧，呼西旦.阿巴拜克力，刘思国，杨启元，施远翔，王六合，陈利苹，李君莲， 陈飞，帕提玛•努合曼，程维疆，何生，郭亚军，王爱菊，方新元，张成成，郭新钢，刘军成，周棕长，王杏芹，赛力克江，韩义忠，舒峰，彭华刚，罗文毅，2016年；

3. 牛结核病病原生态学和流行病学研究，黑龙江省自然科学三等奖，刘思国，司微，于申业，王牟平，陈利苹，2014年；

4. 牛结核病病原生态学和流行病学研究，中国农业科学院自然科学二等奖，刘思国，刘慧芳，司微，王春来，于申业，王牟平，2012年；