Siqi Li，Chunsheng Wang，Yan-Dong Tang，Lei Qin，Tianfeng Chen，Shanghui Wang，Yuanzhe Bai，Xuehui Cai，Shujie Wang

Microorganisms.2023 Jan 8;11(1):160.doi: 10.3390/microorganisms11010160.

Abstract

Streptococcus suis  is an important swine bacterial pathogen that activates macrophages to secrete inflammatory cytokines. Primary porcine alveolar macrophages (PAMs) are inconvenient to obtain, but it is unknown whether immortalized PAM-Tang cells can replace them as a better cell model for the study of the interaction between  S. suis  and macrophages. In this study, the phagocytic integrity, polarization, and pro-inflammatory cytokine secretion of PAM-Tang cells were confirmed by live-cell imaging, electron microscopy, confocal microscopy, and ELISA. Interestingly, the  S. suis  serotype 9 avirulent strain W7119 induced higher levels of adhesion and pro-inflammatory cytokines in PAM-Tang cells than the  S. suis  serotype 2 virulent strain 700794. Prolonged incubation with  S. suis  caused more cytotoxic cell damage, and the virulent strain induced higher levels of cytotoxicity to PAM-Tang cells. The virulent strain also induced higher levels of apoptosis in PAM-Tang cells, as shown by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labeling (TUNEL) assay. In addition, it is the first report of virulent and avirulent  S. suis  inducing PAM-Tang polarization towards pro-inflammatory M1 macrophages and p53- and caspase-dependent apoptosis in PAMs. Taken together, this study contributes to a better understand of interactions between macrophages and  S. suis  isolates of different virulence, and confirms that PAM-Tang cells provide a long-term, renewable resource for investigating macrophage infections with bacteria.

Keywords: PAM-Tang cell line; Streptococcus suis; apoptosis; phagocytosis.