Yuxuan Zheng, Su Li , Shi-Hua Li, Shaoxiong Yu, Qihui Wang , Kehui Zhang, Liang Qu, Yuan Sun, Yuhai Bi, Fuchou Tang, Hua-Ji Qiu, George F Gao

Proc Natl Acad Sci U S A.2022 May 10;119(19):e2201288119.doi: 10.1073/pnas.2201288119. Epub 2022 May 4.

Abstract

SignificanceAfrican swine fever virus (ASFV) causes a severe and highly contagious disease in pigs and wild boars, but no commercial vaccines or antivirals are available currently. Understanding the mutual antagonism between virus and host factors during ASFV infection may facilitate the development of new vaccines and antivirals. Our work profiled transcriptomes of swine macrophages infected with ASFV through single-cell RNA-sequencing technology. Identified dynamic transcriptome events of viral genes provide molecular characteristics of ASFV during infection. Moreover, virus-host interactions imply the regulation pathway of viral replication in host cells, which may guide research on antiviral strategies and dissection of ASFV pathogenesis.

Keywords: African swine fever virus; macrophage; single-cell RNA sequencing; tumor necrosis factor alpha; virus–host interaction.