Wansheng Li,Minhua Li,Hongliang Zhang,Chao Li,Hu Xu,Bangjun Gong,Jun Fu,Zhenyang Guo,Jinmei Peng,Guohui Zhou,Zhijun Tian,Qian Wang

Front Microbiol.2022 Apr 29;13:882112.doi: 10.3389/fmicb.2022.882112.eCollection 2022.

Abstract

A latex microsphere-based immunochromatographic strip (ICS) test was successfully developed for the rapid and sensitive detection of porcine reproductive and respiratory syndrome virus (PRRSV). The PRRSV N protein-specific monoclonal antibody (mAb) 1H4 labeled with latex microspheres was dispensed on a conjugate pad for use as the detector. The same mAb and goat anti-mouse antibody were blotted on a nitrocellulose membrane to generate test and control lines, respectively. The limit of virus detection was approximately 5 × 102.0 median tissue culture infectious dose (TCID50)/ml, and the limit of N protein detection was approximately 15 ng/ml. Other common porcine viruses were tested to evaluate the specificity of the ICS, and positive results were observed for only North American-type PRRSV. A comparison of the strip with a standard diagnostic test (reverse transcriptase polymerase chain reaction, RT-PCR) was also performed, and the results showed that the ICS test exhibited relatively high specificity and sensitivity (90.32 and 73.91%, respectively) and relatively high positive predictive value (PPV) and negative predictive value (NPV; 85 and 82.35%, respectively). These results suggest that the ICS test can be used to rapidly and accurately detect PRRSV and can be suitable for diagnostic applications in the field.

Keywords: N protein; North American-type PRRSV; immunochromatographic strip; latex microsphere; monoclonal antibody; rapid detection.

Copyright © 2022 Li, Li, Zhang, Li, Xu, Gong, Fu, Guo, Peng, Zhou, Tian and Wang.